Composite
Part:BBa_K4409013:Design
Designed by: Kexin Fei Group: iGEM22_Worldshaper-HZ (2022-09-20)
gRNA (CT+GAPDH as guide)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence of Cas12 handle with CT present is utilized to achieve a high sensitivity for the igRNA and Cas12 enzyme. The guide sequence targeting GAPDH dsDNA is chosen because GAPDH dsDNA is easily obtained in high quantity due to the abundant presence of GAPDH genes in cells and can be easily amplified.
Source
The sequence that we designed is based on BBa_K4409000 for Cas12 handle with CT present, and BBA_K4409017 for the guide sequence targeting a DNA sequence of GAPDH.